Castro fired a throw home to catcher John Baker, and home-plate umpire Eric Cooper ruled Rodriguez safe. Renteria called for the challenge, which lasted one minute and five seconds. The ruling on the field was confirmed, tying the game at 2.
The Cubs have made 41 challenges this season. They've won 18 and lost 23.
Rays manager Joe Maddon won a challenge in the first inning.
The Rays had two on with one out in the first when Evan Longoria lined out to third base. Luis Valbuena made the grab and fired to second baseman Javier Baez to double up Ben Zobrist. Second-base umpire Hal Gibson called Zobrist out, because he thought Valbuena's throw beat Zobrist to the bag. That prompted Maddon to leave the dugout and challenge.
After two minutes and 15 seconds, the ruling on the field was overturned.
The Rays have made 36 challenges this season, and 14 were overturned, 12 have stood and 10 have been confirmed.
Bill Chastain is a reporter for MLB.com. Brian Hedger is a contributor to MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.